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US 8,030,544 B2Granted
Methods for plant transformation using spectinomycin selection
AssigneeMonsanto Technology LLC
InventorsBrian Martinell, Michael Petersen, David Somers, Yuechun Wan, Edward Williams, Xudong Ye
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Abstract
The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
Claims10
A method for producing a transgenic plant containing at least two heterologous nucleic acid sequences, comprising:
- (a) providing an explant comprising a first heterologous nucleic acid sequence that confers resistance to an herbicide, an aminoglycoside, or hygromycin;
- (b) inoculating the explant with Agrobacterium comprising a vector which comprises a second heterologous nucleic acid sequence comprising a selectable marker gene conferring spectinomycin resistance;
- (c) co-cultivating the explant with the Agrobacterium to obtain an explant that exhibits spectinomycin resistance;
- and
- (d) regenerating the explant that exhibits spectinomycin resistance into a transgenic plant containing at least the two heterologous nucleic acid sequences;
- wherein the explant is treated with a cytokinin at least during steps (b) and (c).
3 more elements
Dependencies are inferred from the claim text.