Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production
AssigneeCargill, Incorporated
InventorsHolly Jean Jessen, Hans H. Liao, Steven John Gort, Olga V. Selifonova
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Abstract
The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.
Claims55
An isolated nucleic acid molecule, comprising a nucleic acid sequence encoding a protein having at least 95% sequence identity to the protein sequence shown in SEQ ID NO:
- 19, wherein the amino acid corresponding to position 3 of SEQ ID NO:
- 19 is His, the amino acid corresponding to position 24 of SEQ ID NO:
- 19 is Thr, the amino acid corresponding to position 110 of SEQ ID NO:
- 19 is Asn, or the amino acid corresponding to position 113 of SEQ ID NO:
- 19 is Leu, and wherein the protein has beta-alanine aminotransferase (BAAT) activity.
An isolated cell comprising an exogenous nucleic acid molecule encoding SEQ ID NO:
- 19, wherein expression of the nucleic acid molecule results an increase in 3-hydroxypropionic acid (3-HP) in the cell of at least 2-fold as compared to 3-HP expression in the cell in the absence of the exogenous nucleic acid molecule.
Dependencies are inferred from the claim text.