Stabilization of perhydrolases
AssigneeE. I. Du Pont de Nemours and Company
InventorsRobert DiCosimo, Arie Ben-Bassat, Mark S. Payne, Raymond Richard Zolandz
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Abstract
Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
Claims17
A process to stabilize the perhydrolysis activity of an enzyme when present in a formulation comprised of said enzyme and a carboxylic acid ester, the process comprising:
- (a) providing an aqueous formulation comprising
- (i) at least one enzyme structurally classified as a CE-7 enzyme having perhydrolysis activity and a signature motif comprising:
- (A) an RGQ motif at amino acid residues 118-120,
- (B) a GXSQG motif at amino acid residues 179-183, and
- (C) an HE motif at amino acid residues 298-299 when aligned to reference sequence SEQ ID NO:1 using CLUSTALW,
- (ii) at least one excipient, and
- (iii) optionally at least one surfactant;
- (b) spray-drying the aqueous formulation of (a) to produce an enzyme powder comprising said at least one enzyme, said at least one excipient, and optionally said at least one surfactant;
- and
- (c) combining the enzyme powder of (b) with at least one buffer and a carboxylic acid ester to form a formulation, wherein the addition of the at least one buffer to the formulation enhances the stability of the perhydrolysis activity of said at least one enzyme when present in said formulation as compared to the perhydrolase activity of said at least one enzyme when the at least one excipient is not present.
Dependencies are inferred from the claim text.