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US 8,026,412 B2Granted
Soybean MTH1 promoter and its use in constitutive expression of transgenic genes in plants
AssigneeE. I. Du Pont de Nemours and Company
InventorZhongsen Li
Life of this patent
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Abstract
The promoter of a soybean metallothionein protein (MTH1) and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-independent or constitutive manner in plants are described.
Claims14
An isolated polynucleotide comprising
- a nucleotide sequence comprising the sequence set forth in SEQ ID NO:1, wherein said nucleotide sequence is a promoter, or the full-length complement thereof.
An isolated polynucleotide comprising a nucleotide sequence comprising a fragment of SEQ ID NO:1 selected from the group consisting of SEQ ID NOs:2-6, wherein said nucleotide sequence is a promoter, or a full-length complement thereof.
A plant stably transformed with a recombinant expression construct comprising:
- (a) a soybean promoter fragment of SEQ ID NO:1 selected from the group consisting of SEQ ID NOs:2-6 and
- (b) a heterologous nucleic acid sequence operably linked to said promoter fragment;
- wherein said promoter fragment is capable of controlling expression of said heterologous nucleic acid fragment in a plant cell.
Dependencies are inferred from the claim text.