Methods for determining the presence or absence of elite event RF-BN1 in Brassica plant material

A kit for identifying elite event MS-BN1 in a biological sample of a Brassica plant, Brassica plant material, or a product comprising a Brassica plant material, said kit comprising at least two primers, wherein:

• (a) a first primer comprises 19 consecutive nucleotides of nucleotides:
• 1-867 of SEQ ID NO:
• 13;
• or nucleotides 1-771 of SEQ ID NO:
• 36;
• and a second primer comprises 19 consecutive nucleotides of the complement of:
• nucleotides 868-953 of SEQ ID NO:
• 13 or of nucleotides 772-909 of SEQ ID NO:
• 36, or wherein a second primer comprises 19 consecutive nucleotides of SEQ ID NO:
• 1 or the complement thereof;
• or
• (b) a first primer comprises 19 consecutive nucleotides of the complement of nucleotides 181-537 of SEQ ID NO:
• 18 or nucleotides 181-522 of SEQ ID NO:
• 38;
• and a second primer comprises 19 consecutive nucleotides of nucleotides 1-180 of SEQ ID NO:
• 18 or nucleotides 1-180 of SEQ ID NO:
• 38, or wherein a second primer comprises 19 consecutive nucleotides of SEQ ID NO:
• 1, or the complement thereof;
• wherein the first and the second primer amplify a DNA fragment from a nucleic acid present in a biological sample that comprises elite event MS-BN1, and
• wherein the first and the second primer do not amplify said DNA fragment from a nucleic acid present in a biological sample that does not comprise elite event MS-BN1.