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US 8,026,349 B2Granted
Polynucleotide synthesis labeling chemistry
AssigneeDharmacon, Inc.
InventorsStephanie A. Hartsel, Robert J. Kaiser, Michael O. Delaney
Life of this patent
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Abstract
Methods and compositions for making nucleoside phosphoramidites and nucleic acids, including mono-, di-, and polynucleotides, comprising a linker covalently attached to a levulinyl moiety are provided. A levulinyl-protected linking moiety affords an orthogonal approach to modifying a polynucleotide during or after solid phase synthesis with a molecule of interest, for example, a conjugate or a dye.
Claims14
A composition, comprising:
- wherein L comprises a linker;
- Z1 is an oxygen or a sulfur;
- Z2 is an oxygen or a sulfur;
- and S comprises a solid support useful for the synthesis of nucleic acids, wherein the linker is attached to S through a covalent linkage, and the linker comprises a functional group through which the linker can attach to a polynucleotide, and the linker further comprises at least one of an alkane, an alkene, a polyether, a polyalcohol, a polyester, a polyamine, a polyalkylamine, a polyphosphodiseter, a polyethylene glycol, a polypropylene, a propylene glycol, a mixture of ethylene and propylene glycols, or a combination thereof.
1 more element
Dependencies are inferred from the claim text.