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US 8,026,223 B1Granted
Treating malignant tumors with high field strength electroporation of plasmids encoding IL-12
AssigneeUniversity of South Florida
InventorsRichard Heller, Melinda Lee Lucas
Life of this patent
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Abstract
In accordance with the present invention is provided a method of treating a subject having a cancerous tumor. The treatment protocol methodology includes injecting the cancerous tumor with an effective dose of plasmid coding for a therapeutic protein followed by administering electroporation therapy to the tumor, the electroporation therapy includes the administration of at least one high voltage, short duration pulse to the tumor.
Claims18
A method of treating cancer, the method comprising:
- injecting an established cancerous tumor with an effective dose of plasmid coding for IL-12;
- and
- administering electroporation therapy to the established cancerous tumor, the electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.
A method of treating cancer in a subject, the method comprising:
- injecting an established cancerous tumor with an effective dose of plasmid coding for IL-12;
- administering electroporation to the established cancerous tumor using six 1500V/cm pulses, each pulse being 100 microseconds in duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth;
- injecting an effective dose of plasmid coding for IL-12 into the muscle tissue of the subject;
- and
- administering electroporation to the subject intramuscularly using twelve 100V/cm pulses, each pulse being 20 milliseconds in duration.
2 more elements
A method of treating cancer, the method comprising:
- administering a first treatment at time T1, wherein the first treatment comprises injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the tumor at time T1, the first electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond;
- and
- administering a second treatment at time T2, wherein time T2 is a time later than time T1, wherein the second treatment comprises injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the tumor at time T2, the second electroporation therapy comprising the administration of at least one pulse having a field strength of at least 700V/cm and a duration of less than 1 millisecond resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.
A method of treating cancer, the method comprising:
- administering a first treatment on day zero, the first treatment comprising injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the established cancerous tumor, the first electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration;
- and
- administering a second treatment on day seven, the second treatment comprising injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the established cancerous tumor, the second electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.
A method of treating cancer in a subject, the method comprising:
- administering a first treatment on day zero, the first treatment comprising injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the established cancerous tumor, the first electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration;
- administering a second treatment on day seven, the second treatment comprising injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the established cancerous tumor, the second electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth;
- injecting an effective dose of plasmid coding for IL-12 into the muscle tissue of the subject;
- and
- administering electroporation therapy to the subject intramuscularly using twelve pulses delivered at 100V/cm at 20 millisecond duration.
2 more elements
A method of treating cancer in a subject, the method comprising:
- administering a first treatment on day zero, the first treatment comprising injecting an established cancerous tumor with a first effective dose of plasmid coding for IL-12 and administering a first electroporation therapy to the established cancerous tumor, the first electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration;
- administering a second treatment on day four, the second treatment comprising injecting the established cancerous tumor with a second effective dose of plasmid coding for IL-12 and administering a second electroporation therapy to the established cancerous tumor, the second electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration;
- and
- administering a third treatment on day seven, the third treatment comprising injecting the established cancerous tumor with a third effective dose of plasmid coding for IL-12 and administering a third electroporation therapy to the established cancerous tumor, the third electroporation therapy comprising the administration of six pulses delivered at 1500V/cm at 100 microseconds pulse duration resulting in the regression of the established cancerous tumor and metastases and the inhibition of new cancerous tumor growth.
1 more element
Dependencies are inferred from the claim text.