Agglutination inhibition assay method and reagent
AssigneeSekisui Medical Co., Ltd.
InventorsTadaaki Yoshida, Hiroshi Takahashi
Life of this patent
Estimating the term…
Abstract
Provided are an agglutination-inhibition assay and a reagent for agglutination-inhibition assay, which can be used for measuring a ligand in a sample at high sensitivity in a wide range from the low-concentration range to the high-concentration range and have good reproducibility of measurement. Specifically, provided are an agglutination-inhibition assay and a reagent for agglutination-inhibition assay, in which used are an insoluble carrier particle carrying a ligand, a specific receptor in the free-form and an insoluble carrier particle carrying a specific receptor which binds to a different site on the ligand than the receptor in the free-form.
Claims20
A method for measurement of agglutination inhibition comprising,
- mixing a sample containing a ligand to be measured, having multiple receptor binding sites, with the following:
- a first insoluble carrier particle that preliminarily carries a plurality of the ligand or a plurality of a ligand-like substance,
- a first free-form receptor that binds specifically to a site on the ligand or the ligand-like substance, and
- a second insoluble carrier particle that carries a second receptor that is different from the first free-form receptor and binds specifically to a different site on the ligand or the ligand-like substance than the first free-form receptor;
- and
- measuring an agglutination inhibition reaction of the particles responsive to an amount of the ligand to be measured in the sample.
A reagent for agglutination-inhibition assay comprising the following:
- a first insoluble carrier particle that carries a plurality of a ligand or a plurality of a ligand-like substance,
- a first free-form receptor that binds specifically to a site on the ligand or the ligand-like substance, and
- a second insoluble carrier particle that carries a second receptor that is different from the first free-form receptor and binds specifically to a different site on the ligand or the ligand-like substance than the first free-form receptor,
- the assay being for measurement of an agglutination inhibition reaction of particles responsive to an amount of the ligand to be measured in a sample.
Dependencies are inferred from the claim text.