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US 8,026,093 B2Granted
Particulate substrates for improved recovery of microbes
AssigneeThe Regents of the University of California
InventorsRosalind Tung, George W. Chang
Life of this patent
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Abstract
Specially modified microbial growth surfaces improve bacterial recovery or counts when testing for the presence or absence of microbial cells or performing microbial enumerations.
Claims20
A method for improved recovery of a target bacterium from a dilute aqueous sample, comprising the steps of:
- in a vessel, forming a mixture of determined amounts of a bacterial growth nutrient, a sterile particulate substrate, and an aqueous sample comprising a target bacterium at a concentration of less than 103/mL, wherein the substrate is chemically and nutritively inert to the bacterium yet is determined to promote recovery of the bacterium in the mixture;
- incubating the mixture in the vessel under conditions wherein but for the presence of the substrate, the bacterium is unable to utilize the nutrient and multiply sufficiently to effect a detectable change in the mixture;
- and
- detecting a resultant change in the mixture, wherein the change is diagnostic of the bacterium utilizing the nutrient and multiplying sufficiently to recover the bacterium and to effect the change in the mixture.
1 more element
A method for improved recovery of a target bacterium from a dilute aqueous sample, comprising the steps of:
- in a vessel, forming a mixture of determined amounts of a bacterial growth nutrient, a sterile particulate substrate, and an aqueous sample comprising a target bacterium at a concentration of less than 103/mL, wherein the substrate is chemically and nutritively inert to the bacterium yet is determined to promote recovery of the bacterium in the mixture;
- incubating the mixture in the vessel under conditions wherein but for the presence of the substrate, the bacterium is unable to utilize the nutrient and multiply sufficiently to effect a detectable change in the mixture;
- and
- detecting a resultant change in the mixture, wherein the change is diagnostic of the bacterium utilizing the nutrient and multiplying sufficiently to recover the bacterium and to effect the change in the mixture,
- wherein the sample contains a chlorine residual which eradicates most bacteria, but leaves 1-10 chlorine-injured E. coli or other coliform bacteria cells per 100-mL sample volume.
2 more elements
Dependencies are inferred from the claim text.