Modified chondroitin synthase polypeptide and crystal thereof
AssigneeSeikagaku Corporation
InventorsYoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
Life of this patent
Estimating the term…
Abstract
Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.
Claims12
An isolated or purified nucleic acid that encodes a polypeptide having chondroitin synthase activity
- (i) that consists of the polypeptide of SEQ ID NO:
- 2;
- (ii) that consists of the amino acid sequence of SEQ ID NO:
- 2 except that it includes deletion, substitution, or addition of one or two amino acids within SEQ ID NO:
- 2;
- (iii) that consists of a polypeptide having no less than 95% homology to the amino acid sequence of SEQ ID NO:
- 2;
- or
- (iv) that comprises the amino acid sequence of SEQ ID NO:
- 2, but which does not include 57 amino acid residues from the N-terminal of K4CP of SEQ ID NO:4.
Dependencies are inferred from the claim text.