Synthetic gene control region
AssigneeMerck Sharp & Dohme Corp.
InventorsJanine Bryan, Michelle K. Brownlow, Loren D. Schultz, Maria C. Losada, Kathrin Ute Jansen, Myra Kurtz
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Abstract
The present invention provides a synthetic gene control region which comprises a gene regulatory sequence comprising a binding site for a gene regulatory protein of a yeast strain, and a promoter from filamentous fungal strain located downstream of the gene regulatory sequence; wherein the promoter can be recognized by the general transcription factors and RNA polymerase of the yeast strain; wherein the gene regulatory sequence is capable of regulating transcription initiated by the filamentous fungal promoter in the yeast strain.
Claims18
A synthetic gene control region comprising, a synthetic gene regulatory sequence comprising a binding site for a gene regulatory protein of a yeast strain, and a promoter from a filamentous fungal strain located downstream of the synthetic gene regulatory sequence;
- wherein the binding site is a binding site for GAL4 protein of S. cerevisiae, and the promoter is a promoter for the XynA gene of Penicillium purpurogenum, wherein the promoter can be recognized by the general transcription factors and RNA polymerase of the yeast strain;
- wherein the synthetic gene regulatory sequence is capable of regulating the transcription initiated by a filamentous fungal promoter in the yeast strain.
A DNA expression vector comprising, a synthetic gene control region, wherein the synthetic gene control region comprises a sequence selected from the group consisting of SEQ ID NO:21, 22, 23, 24, 25, and 26;
- a coding sequence encoding a protein, polypeptide, or peptide under the control of the control region, and an yeast selection marker.
A yeast strain comprising a DNA expression vector wherein the DNA expression vector comprises, a synthetic gene control region which comprises a sequence selected from the group consisting of SEQ ID NO:21, 22, 23, 24, 25, and 26, a a coding sequence encoding a protein, polypeptide, or peptide under the control of the control region, and an yeast selection marker.
Dependencies are inferred from the claim text.