Systems and methods for detecting target analytes
AssigneeFabrico Technology, Inc.
InventorsRobert C Chin, Gregory F. Lopreato
Life of this patent
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Abstract
A method of detecting a target substance includes contacting the target substance with a substrate. The substrate has a first receptor bound to the substrate. The target substance binds to the first receptor. The method further includes contacting a second receptor with the substrate. The second receptor is associated with the target substance. The second receptor is biotinylated. The method also includes contacting an anti-biotin antibody conjugated paramagnetic particle with the substrate.
Claims20
A method of detecting an endogenous human target, the method comprising:
- applying the endogenous human target to a substrate, the substrate having a first receptor attached to the substrate, the first receptor specific to the endogenous human target;
- applying non-specific anti-human antibodies conjugated to paramagnetic particles to the substrate, the non-specific anti-human antibodies binding to the endogenous human target;
- washing the substrate;
- and
- detecting the paramagnetic particles to detect the endogenous human target.
A method of detecting an endogenous human target, the method comprising:
- applying the endogenous human target to a substrate, the substrate having a first receptor attached to the substrate, the first receptor specific to the endogenous human target;
- applying non-specific anti-human antibodies to the substrate, the non-specific anti-human antibodies binding to the endogenous human target;
- applying conjugated paramagnetic particles to the substrate, the conjugated paramagnetic particles adapted to respond to the non-specific anti-human antibody;
- washing the substrate;
- and
- detecting the paramagnetic particles to detect the endogenous human target.
A method of detecting an endogenous human target, the method comprising:
- applying the endogenous human target to a substrate, the substrate including an immobilized antibody specific to the endogenous human target, the endogenous human target binding to the immobilized antibody;
- applying paramagnetic particles conjugated to non-specific anti-human antibodies, the non-specific anti-human antibodies binding to the endogenous human target, whereby the paramagnetic particles are immobilized relative to the substrate washing the substrate;
- applying an electromagnetic field across the substrate, the paramagnetic particles having an influence on the electromagnetic field;
- and
- determining the amount of the endogenous human target based on a correlation associated with the influence of the paramagnetic particles on the electromagnetic field.
Dependencies are inferred from the claim text.