Differential detection of multimeric and monomeric forms of multimer-forming polypeptides
AssigneePeoplebio, Inc.
InventorsSeong Soo Alexander An, Kun Taek Lim, Hyun Jung Oh
Life of this patent
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Abstract
A method for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide in a biosample includes (a) contacting the biosample to a capturing antibody recognizing an epitope on the multimer-forming polypeptide to capture the monomeric form, multimeric form or monomeric and multimeric forms; (b) contacting the monomeric form, multimeric form or monomeric and multimeric forms captured to a detecting antibody recognizing an epitope identical to or overlapped with the epitope of step (a); and (c) detecting the formation of a multimeric form-detection antibody complex.
Claims17
A method for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide in a biosample, comprising:
- (a) contacting the biosample to a capturing antibody recognizing an epitope on the multimer-forming polypeptide to capture the monomeric form, multimeric form or monomeric and multimeric forms;
- (b) contacting the monomeric form, multimeric form or monomeric and multimeric forms captured by the capturing antibody to a detecting antibody recognizing an epitope which is overlapped with, but not identical to the epitope of (a);
- wherein said detecting antibody is linked to a label generating a detectable signal;
- and said epitope recognized by the capturing antibody or detecting antibody is not repeated in the monomeric form of a multimer-forming polypeptide;
- and
- (c) detecting the formation of a multimeric form-detection antibody complex by measuring a signal generated from the label linked to the detecting antibody.
Dependencies are inferred from the claim text.