Use of silica material in an amplification reaction
AssigneeRoche Molecular Systems, Inc.
InventorsJudith Pinsl-Ober, Peter Wenzig, Ralf Schoenbrunner, Patrick O'Donnell, Erich Kyger, Khushbeer Malhotra, Kurt Weindel, Knut Bartl
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Abstract
This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.
Claims11
A method for the purification and amplification of a target nucleic acid from a biological sample comprising said target nucleic acid, said method comprising the steps of:
- a) adding a material comprising magnetic glass particles with an unmodified silica surface to said sample to bind said target nucleic acid to said material,
- b) separating said material from said sample,
- c) eluting said target nucleic acid from said material, and
- d) amplifying said target nucleic acid in the presence of at least 50% of the material added in step a), wherein said target nucleic acid is detected during amplification and wherein said magnetic glass particles are manufactured by the sol-gel method.
Dependencies are inferred from the claim text.