Assessment of oocyte competence
AssigneeYale University
InventorsDagan Wells, Pasquale Patrizio
Life of this patent
Estimating the term…
Abstract
Methods are provided for evaluating an oocyte for fertilization and implantation. For example, methods are provided for determining whether an oocyte expresses, or does not express, one or more of a group of markers identified as differently expressed between chromosomally normal and chromosomally abnormal oocytes. Also provided, for example, are methods for determining whether a cumulus cell expresses, or does not express, one or more of a group of markers identified as differently expressed between cumulus cells associated with chromosomally normal oocytes and cumulus cells associated with chromosomally abnormal oocytes. Methods are provided for the detection of marker expression of differentially expressed genes at the RNA level, as well as at the protein level.
Claims48
A method of evaluating the competence of a mammalian oocyte for implantation comprising:
- (i) determining in a sample the level of marker expression of at least one nucleic acid selected from the group of nucleic acids exemplified by SEQ ID NOS:
- 19, 25, 33, 38, and 43, and
- (ii) comparing the level of marker expression in the sample with a control or reference standard, wherein detecting differential marker expression between the sample and the control is indicative of the competence of the oocyte for implantation.
A method of evaluating the competence of a mammalian oocyte for implantation comprising:
- (i) determining in a sample the level of marker expression of at least one nucleic acid selected from the group of nucleic acids exemplified by SEQ ID NOS:
- 184, 187, 189, 191, and 230, and
- (ii) comparing the level of marker expression in the sample with a control or reference standard, wherein detecting differential marker expression between the sample and the control is indicative of the competence of an oocyte for implantation.
A method of evaluating the competence of a mammalian oocyte for fertilization comprising:
- (i) determining in a sample the level of marker expression of at least one nucleic acid selected from the group of nucleic acids exemplified by SEQ ID NOS:
- 19, 25, 33, 38, and 43 and
- (ii) comparing the level of marker expression in the sample with a control or reference standard, wherein detecting differential marker expression between the sample and the control is indicative of the competence of the oocyte for implantation.
A method of evaluating the competence of a mammalian oocyte for fertilization comprising:
- (i) determining in a sample the level of marker expression of at least one nucleic acid selected from the group of nucleic acids exemplified by SEQ ID NOS:
- 184, 187, 189, 191, and 230, and
- (ii) comparing the level of marker expression in the sample with a control or reference standard, wherein detecting differential marker expression between the sample and the control is indicative of the competence of an oocyte for fertilization.
Dependencies are inferred from the claim text.