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US 8,026,058 B2Granted
PCR hot start by magnesium sequestration
AssigneeRoche Diagnostics Operations, Inc.
InventorsWaltraud Ankenbauer, Dieter Heindl, Frank Laue
Life of this patent
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Abstract
The present invention is directed to a synthetic peptide having a length of not more than 30 amino acids comprising a divalent cation binding site. Such a peptide according to the present invention is part of a composition for nucleic acid amplification and provides for a so-called hot start effect.
Claims8
A synthetic peptide consisting of a divalent cation binding site selected from the group consisting of H-DIETDIET-NH2 (SEQ ID NO:
- 9), H-DIETDIETDIET-NH2 (SEQ ID NO:
- 8), and H-FDGDFDGD-NH2 (SEQ ID NO:
- 10).
A composition comprising a synthetic peptide consisting of a divalent cation binding site selected from the group consisting of H-DIET-NH2 (SEQ ID NO:
- 11), H-DIETDIET-NH2 (SEQ ID NO:
- 9), H-DIETDIETDIET-NH2 (SEQ ID NO:
- 8), and H-FDGDFDGD-NH2 (SEQ ID NO:
- 10), a thermostable DNA polymerase, a divalent cation, deoxynucleotides, and a buffer.
1 more element
Dependencies are inferred from the claim text.