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US 8,026,054 B2Granted
Antibodies against cells of fetal origin
AssigneeThe Board of Trustees of the University of Illinois
InventorsArun Sharma, Sherman Elias
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Abstract
This invention relates to antibodies that specific bind to fetal CD36+ cells in preference to binding to maternal CD36+ cells and methods for using these antibodies to detect and separate fetal cells from adult biological fluids including maternal peripheral blood.
Claims6
A method for producing antibodies specific for fetal CD36+ cells, comprising the steps of:
- (a) stimulating CD34+ adult cells isolated from a biological fluid to express CD36;
- (b) isolating differentiated adult cells stimulated according to step (a);
- (c) stimulating CD34+ fetal cells isolated from fetal tissue to express CD36;
- (d) isolating differentiated fetal cells stimulated according to step (c);
- (e) isolating total RNA from the adult cells of step (b) and the fetal cells of step (d);
- (f) analyzing said isolated total RNA of step (e) to identify genes encoding cellular proteins that are significantly expressed by the isolated fetal cells, but not significantly expressed by the isolated adult cells;
- (g) obtaining cellular proteins or peptides thereof that are encoded by the genes identified in step (f);
- and
- (h) preparing antibodies against the cellular proteins or peptides thereof obtained in step (g) using the cellular proteins or peptides thereof as antigens.
6 more elements
Dependencies are inferred from the claim text.