Loading patent…
AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsMarc Eichmeyer, Greg Nitzel, Merrill Schaeffer
Estimating the term…
An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
An immunogenic composition comprising 4 μg to 400 μg of recombinant PCV2 ORF2 protein and a polymer of acrylic or methacrylic acid, wherein said immunogenic composition is effective for providing protective immunity and lessening the severity of one or more clinical symptoms caused by a PCV2 infection after a single dose thereof, in comparison to an animal not receiving said immunogenic composition and wherein said one or more clinical symptoms are selected from the group consisting of wasting, paleness of the skin, unthriftiness, respiratory distress, diarrhea, icterus, jaundice, lung lesions, nasal shedding, cough, diarrhea, and combinations thereof.
A kit comprising a container and an instruction manual:
Dependencies are inferred from the claim text.