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US 8,021,882 B2Granted
Production of neural protective and regenerative factors from stem cells and treatment of nervous system conditions therewith
OwnerBrian H. Johnstone, Keith Leonard March +1inventors
InventorsBrian H. Johnstone, Keith Leonard March, Yansheng Du
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Abstract
A method for producing stem cell conditioned media for treatment of neurological insults is provided herein. By providing for the culture of adipose stem cells, and collecting the supernatants thereof, the supernatants have been shown to effect biological activity in preventing neural death when the supernatants are administered to a patient that has or is about to be subjected to a neural insult.
Claims11
A method of producing a stem cell conditioned media, comprising the steps of:
- a. providing at least one human adipose stem cell;
- b. providing at least one medium for culturing the stem cell;
- c. culturing the at least one human adipose stem cell in the at least one medium;
- d. switching the medium to a growth-factor free basal media for conditioning the at least one human adipose stem cell in either normoxic or hypoxic conditions for 72 hours;
- and
- e. collecting cell supernatants effective to treat neural insults.
3 more elements
A method of producing a stem cell conditioned media, comprising the steps of:
- a. providing at least one human or rat adipose stem cell harvested from an individual to be treated for a neural insult, the at least one stem cell comprising an adipose stem cell harvested from a lipoaspirate;
- b. providing at least one medium for culturing the stem cell;
- c. culturing the at least one human or rat adipose stem cell in the at least one medium;
- d. switching the medium to a growth-factor free basal media for conditioning the at least one human or rat adipose stem cell in either normoxic or hypoxic conditions for 72 hours;
- and
- e. collecting cell supernatants effective to treat neural insults and introducing the cell supernatants to the individual in a therapeutic dose.
3 more elements
Dependencies are inferred from the claim text.