Microorganisms for therapy
AssigneeGenelux Corporation
InventorsAladar A. Szalay, Tatyana Timiryasova, Yong A. Yu, Qian Zhang
Life of this patent
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Abstract
Methods for production of tumor-specific antibodies are provided. The methods employ microorganisms that are designed to accumulate in immunoprivileged tissues and cells, such as in tumors and other proliferating tissue and in inflamed tissues, compared to other tissues, cells and organs, so that they exhibit relatively low toxicity to host organisms. The microorganisms also are designed or modified to result in leaky cell membranes of cells in which they accumulate, resulting in production of antibodies reactive against proteins and other cellular products and also permitting exploitation of proliferating tissues, particularly tumors, to produce selected proteins and other products.
Claims26
A method for the production of antibodies against tumor antigens encoded by the tumor cells and produced in the tumor of a tumor-bearing non-human animal, comprising:
- (a) producing a non-human animal that bears a tumor that expresses tumor antigens against which antibodies are to be produced, wherein tumor cells in the tumor encode and produce the tumor antigens;
- (b) administering a microorganism to the tumor-bearing non-human animal, wherein the microorganism is an attenuated oncolytic virus, which does not encode a tumor antigen encoded by the tumor cells and which accumulates and replicates in the tumor cells, whereby the animal produces antibodies against the tumor's antigens;
- and
- (c) isolating from the blood or serum of the non-human animal, antibodies against the tumor's antigens encoded by and produced by cells in the tumor.
A method for the production of antibodies against tumor antigens encoded by the tumor cells and produced in the tumor of a tumor-bearing animal, comprising:
- (a) administering a microorganism to a tumor-bearing animal, wherein:
- tumor cells in the tumor encode and produce tumor antigens;
- the microorganism is an LIVP strain of vaccinia virus, which is attenuated and accumulates and replicates in the tumor cells and does not encode a tumor antigen encoded by the tumor cells;
- and
- the microorganism does not accumulate to toxic levels in organs and tissues that do not comprise tumor cells, whereby the animal produces antibodies against the tumor antigens;
- and
- (b) isolating antibodies against the tumor antigens encoded by and produced by cells in the tumor from the blood or serum of the animal.
Dependencies are inferred from the claim text.