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US 8,021,613 B2Granted
System and method for self-referencing a sensor in a micron-sized deep flow chamber
AssigneeCorning Incorporated
InventorsNorman H. Fontaine, Prantik Mazumder, Mark A. Quesada, Eric J. Mozdy, Po Ki Yuen
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Abstract
A system and method are described herein for self-referencing a sensor that is used to detect a biomolecular binding event and/or kinetics which occur in a sample solution flowing along side a reference solution in a micron-sized deep flow channel.
Claims14
A system comprising:
- a sensor;
- a micron-sized deep flow channel in which a sample solution and a reference solution flow side-by-side to one another in a single sensing region of said sensor, wherein the single sensing region is divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with the sample solution and the reference solution that flow side-by-side to one another in a longitudinal direction within the micron-sized deep flow channel;
- an interrogation system configured to direct an input optical beam at said sensor and receiving an output optical beam from said sensor;
- said interrogation system including a camera configured to convert the output optical beam to an electrical signal representative of an output image;
- a computer configured to analyze the output image to determine a detection signal associated with the sample solution flowing in the detection region of said sensor and to determine a reference signal associated with the reference solution flowing in the reference region of said sensor;
- and
- said computer configured to subtract the reference signal from the detection signal to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions are reduced in the corrected detection signal.
4 more elements
A system comprising:
- a sensor;
- a micron-sized deep flow channel in which a sample solution and a reference solution flow side-by-side to one another in a single sensing region of said sensor, wherein the single sensing region is divided into a detection region and a reference region which are contiguous to one another and which are respectively interfaced with the sample solution and the reference solution that flow side-by-side to one another in a longitudinal direction within the micron-sized deep flow channel;
- an interrogation system configured to direct an input optical beam at said sensor and receiving an output optical beam from said sensor;
- said interrogation system including an optical detector configured to convert the output optical beam to separate electrical signals, one each representing a detection signal associated with the sample solution flowing in the detection region of said sensor and another representing a reference signal associated with the reference solution flowing in the reference region of said sensor;
- and
- a computer/electrical circuit configured to subtract the reference signal from the detection signal to generate a corrected detection signal, wherein uncertainties in the detection signal due to environmental conditions are reduced in the corrected detection signal.
3 more elements
Dependencies are inferred from the claim text.