Method for the preparation of dermal papilla tissue employing mesenchymal stem cells

A method for the preparation of a dermal papilla tissue, which comprises the steps of:

• 1) culturing mesenchymal stem cells in a first culture medium, said first culture medium being an animal cell culture medium and containing 600 to 1,900 mg/l of amino acids and 12 to 36 mg/l of vitamins;
• 2) culturing the cells obtained in step (1) in a second culture medium which contains 2,000 to 3,000 mg/l of amino acids and 40 to 60 mg/l of vitamins, wherein the second culture medium is supplemented with one or more growth factors selected from the group consisting of hepatocyte growth factor, epidermal growth factor and nerve growth factor, and wherein the second culture medium is free of serum;
• 3) adding trypsin or collagenase to the culture obtained in step (2) to allow a lump of cells to detach from the culture dish and undergo auto-aggregation to form a dermal papilla tissue;
• and
• 4) harvesting the dermal papilla tissue obtained in step (3) and culturing the tissue in the second culture medium.