Monolithic column chromatography
AssigneeQuest Diagnostics Investments Incorporated
InventorsNigel Clarke, Mildred M. Goldman
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Abstract
Provided herein are methods of liquid column chromatography in which preparative chromatography is performed in-line with analytical chromatography. In particular aspects a monolithic preparative column is used to purify an analyte of interest from a mixture of other substances by applying the mixture to the column, reversing the flow through the column to elute the analyte, which is applied to an analytical column provided in-line with the preparative column. In other aspects, a single monolithic column is used to perform both the preparative chromatography and analytical chromatography steps in succession. In another aspect, a chromatography system is provided to perform preparative and analytical chromatography using a single monolithic column.
Claims11
A chromatographic method for detecting one or more analytes in a body fluid sample containing a mixture of other substances, said method comprising:
- a) separating said one or more analytes from said other, substances in a mixture using a first chromatography column comprising a monolithic sorbent having macropores and mesopores, using a first mobile phase under conditions such that said one or more analytes are retained on said first column and other substances are removed;
- wherein said one or more analytes retained on said first column have a molecular weight of less than about 5,000 daltons,
- b) eluting said one or more retained analytes by applying a second mobile phase to the column,
- c) directing the eluted one or more analytes to a second chromatography column in-line with said first column for further separation, and
- d) detecting said one or more analytes following the separation in step c).
Dependencies are inferred from the claim text.