Integrated systems and methods for diversity generation and screening

A diversity generation device comprising:

• a fragmentation module configured to fragment parental nucleic acids to produce nucleic acid fragments;
• and
• a programmed thermocycler operably coupled to the fragmentation module and configured to receive the nucleic acid fragments from the fragmentation module and to reassemble the nucleic acid fragments to produce shuffled nucleic acids,
• wherein the programmed thermocycler comprises a thermocycler operably coupled to a computer,
• wherein the computer comprises one or more instruction sets for:
• calculating one or more crossover regions between two or more parental nucleic acid sequences of the parental nucleic acids;
• using the calculated one or more crossover regions to select the parental nucleic acids for use in the fragmentation module;
• and
• performing one or more calculations based on one or more theoretical predictions of a nucleic acid melting temperature or based on one or more sets of empirical data, the one or more sets of empirical data comprising a comparison of one or more nucleic acid melting temperatures.

A diversity generation device comprising:

• a fragmentation module configured to fragment parental nucleic acids to produce nucleic acid fragments;
• and
• a programmed thermocycler operably coupled to the fragmentation module and configured to receive the nucleic acid fragments from the fragmentation module and to reassemble the nucleic acid fragments to produce shuffled nucleic acids,
• wherein the programmed thermocycler comprises a thermocycler operably coupled to a computer,
• wherein the computer comprises one or more instruction sets for:
• calculating one or more crossover regions between two or more parental nucleic acid sequences of the parental nucleic acids;
• using the calculated one or more crossover regions to select the parental nucleic acids for use in the fragmentation module;
• and
• calculating the one or more crossover regions comprise instructions for identifying sequence windows having a melting temperature greater than a predetermined threshold, the sequence windows are separated by no more than two nucleotides.