Method for confirming positions on which probes are immobilized in nucleic acid array

A method for confirming immobilization conditions of nucleic acid probes immobilized on a nucleic acid array, comprising the steps of:

• (a) defining the number of zones to which the nucleic acid probes immobilized on the nucleic acid array belong and one or more given pieces of identification information;
• (b) calculating X using the following formula:
• X={log(N+1)M}+1
• (wherein N represents the number of the pieces of identification information, and M represents the number of the zones to which the nucleic acid probes immobilized on the nucleic acid array belong),
• defining a number of the integer portion of X as the number of nucleic acid arrays required for confirming the immobilization conditions, Xa, and allocating non-overlapping numerical values
• (Y), which are expressed by notation system of base N+1, and which have the same digit number as Xa, to the respective nucleic acid probes belonging to the zones;
• (c) preparing complementary nucleic acid molecules comprising nucleotide sequences complementary to all or a part of nucleotide sequences of the nucleic acid probes, preparing groups of nucleic acid samples corresponding to the number of nucleic acid arrays (Xa) by mixing the complementary nucleic acid molecules based on the number of every digit of each of the allocated numerical values (Y), and preparing nucleic acid arrays (the number:
• Xa);
• (d) contacting each of the nucleic acid arrays (the number:
• Xa) prepared in the step (c) with the corresponding group of the nucleic acid samples to detect signals derived from hybrids between the nucleic acid probes immobilized on the nucleic acid arrays and the complementary nucleic acid molecules;
• (e) matching patterns of expression of the signals detected to patterns of the numerical values (Y) allocated;
• and
• (f) confirming the immobilization conditions when the patterns of expression match the patterns of the numeric values (Y).