Method, arrangement, and software for optimizing the image quality of movable subjects imaged with a microscope
AssigneeLeica Microsystems CMS GmbH
InventorFrank Olschewski
Life of this patent
Estimating the term…
Abstract
A method and an arrangement for optimizing the image quality of movable subjects imaged with a microscope system are proposed. The microscope system encompasses at least one objective that defines an image window. Motions of the subjects being observed are captured in the image frame. A computer system, having a means for determining a respective displacement vector field from a comparison of the respective pixels of two chronologically successive images, generates a trajectory from the synopsis of the displacement vector field of all the acquired images. A means for applying an operation to the image data along a trajectory is also provided.
Claims10
A method for optimizing image quality in a microscope system, comprising the following steps:
- optically acquiring images of a subject under a microscope by a detector unit, each image having a plurality of pixels;
- storing the images optically acquired by the detector unit in a first image memory;
- for each two chronologically successively acquired images comparing two chronologically successively acquired values detected for each pixel of the detector unit to determine a respective displacement vector field between the two chronologically successively acquired images;
- identifying a trajectory for each pixel of the acquired images from respective displacement vector fields determined for each two chronologically successively acquired images;
- storing trajectory data obtained from the images optically acquired by the detector unit in a trajectory memory;
- applying an operation to the images optically acquired by the detector unit along the identified trajectory;
- and
- storing the images created by filtering of the images from the first image memory with the trajectory data from the trajectory memory in a second image memory.
An arrangement for optimizing image quality in a microscope system, comprising:
- at least one objective defining an image window, a detector unit for optically acquiring images of a subject under a microscope, each image optically acquired by the detector unit having a plurality of pixels, and
- a computer system comprising:
- a means for determining a respective displacement vector field from a comparison of each two chronologically successively acquired images comparing two chronologically successively acquired values detected for each pixel of the detector unit,
- a means for identifying a trajectory for each pixel of the images optically acquired by the detector unit from respective displacement vector fields determined for each two chronologically successively acquired images,
- a means for applying an operation to the images optically acquired by the detector unit along the identified trajectory,
- a first image memory storing the images optically acquired by the detector unit,
- a trajectory memory storing trajectory data obtained from the images optically acquired by the detector unit, and
- a second image memory storing the images created by filtering of the images from the first image memory with the trajectory data from the trajectory memory.
Dependencies are inferred from the claim text.