NMR-detecting cell, NMR-measuring method, and NMR-measuring apparatus
AssigneeJapan Advanced Institute of Science and Technology
InventorShinya Ohki
Life of this patent
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Abstract
An object of the present invention is to provide an effective method for measuring by NMR in real time, an NMR-detecting cell for measurement of NMR, and an NMR-measuring apparatus, and specifically, there has been found out a method for measuring NMR wherein a measurement sample is immobilized in a medium in an NMR-detecting cell that is an NMR detection part within an NMR-measuring magnet, and then measured by solution NMR in real time under the condition that the external environment around the immobilized measurement sample is consecutively changing.
Claims17
A method for high throughput screening candidate interactive substances comprising:
- introducing a measurement sample in a medium into an analytical column;
- immobilizing the measurement sample on a solid-phase carrier in the analytical column in an NMR-detecting cell;
- supplying the immobilized measurement sample with a candidate interactive substance through a solution-introducing pipe;
- applying a magnetic field to the NMR-detecting cell;
- measuring the interaction between the measurement sample and the candidate interactive substance by solution NMR;
- supplying a solvent capable of dissociating only the association between the measurement sample and the candidate interactive substance through the solution-introducing pipe to release the candidate interactive substance from the measurement sample;
- discharging the candidate interactive substance from the NMR-detecting cell through a solution-discharging pipe;
- and
- repeating the first supplying step, the measuring step, and the second supplying step.
A method for carrying out solution NMR measurement in real time comprising:
- introducing a measurement sample in a medium into an analytical column in a NMR-detecting cell;
- immobilizing the measurement sample on a solid-phase carrier in the analytical column in the NMR-detecting cell;
- applying a magnetic field to the NMR-detecting cell;
- supplying a solution containing external environment-changing factors through a solution-introducing pipe in order to change consecutively the external environment around the immobilized measurement sample;
- and
- measuring the measurement sample by solution NMR during the supplying step.
A solution NMR apparatus comprising:
- an analytical column having an introducing side and a discharging side, provided with filters on the introducing side and/or on the discharging side, and filled with a solid-phase carrier capable of immobilizing a measurement sample in a medium;
- a solution-introducing pipe, operably associated with the introducing side, capable of supplying a solution for changing the external environment around the immobilized measurement sample or a candidate interactive substance;
- a solution-discharging pipe, operably associated with the discharging side, capable of discharging the solution or the candidate interactive substance;
- and
- a tube operably connectable to the solution-introducing pipe and the analytical column on the introducing side.
An NMR-detecting cell for a solution NMR apparatus comprising:
- a solution-introducing pipe extending outside an NMR measuring magnet;
- a solution-discharging pipe extending outside the NMR measuring magnet;
- and
- an analytical column operably connectable to the solution-introducing pipe on an introducing side and the solution-discharging pipe on a discharging side, provided with filters on the introducing side and/or on the discharging side, and further filled with a solid-phase carrier.
A method for solution NMR-measuring comprising:
- introducing a measurement sample in a medium into an analytical column,
- immobilizing the measurement sample in the medium in an NMR-detecting cell
- applying a magnetic field to the NMR-detecting cell;
- and
- measuring the immobilized measurement sample by solution NMR under the condition that the external environment around the immobilized measurement sample is consecutively changing.
Dependencies are inferred from the claim text.