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US 8,013,215 B2Granted
Production of arachidonic acid in oilseed plants
AssigneeE.I. du Pont de Nemours and Company
InventorsHoward Glenn Damude, Anthony J. Kinney
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Abstract
Oilseed plants which have been transformed to produce arachidonic acid, recombinant constructs used in such transformations, methods for producing arachidonic acid in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.
Claims11
A transgenic oilseed plant comprising:
- (a) a first recombinant construct comprising at least one regulatory sequence operably linked to a polynucleotide encoding at least one delta-8 desaturase polypeptide;
- (b) a second recombinant construct comprising at least one regulatory sequence operably linked to a polynucleotide encoding at least one delta-9 elongase polypeptide;
- (c) a third recombinant construct comprising at least one regulatory sequence operably linked to a polynucleotide encoding at least one delta-5 desaturase polypeptide;
- and
- (d) decreased delta-15 desaturase activity relative to a wild-type or non-transformed plant;
- wherein the transgenic oilseed plant produces mature seeds in which the total seed fatty acid profile comprises at least 10.0% arachidonic acid and less than 5% total omega-3 fatty acids having at least eighteen carbon atoms and at least four double bonds.
3 more elements
A transgenic oilseed plant comprising:
- (a) a first recombinant construct comprising at least one regulatory sequence operably linked to a polynucleotide encoding at least one delta-8 desaturase polypeptide;
- (b) a second recombinant construct comprising at least one regulatory sequence operably linked to a polynucleotide encoding at least one delta-9 elongase polypeptide;
- (c) a third recombinant construct comprising at least one regulatory sequence operably linked to a polynucleotide encoding at least one delta-5 desaturase polypeptide;
- and
- (d) decreased delta-15 desaturase activity relative to a wild-type or non-transformed plant;
- wherein the transgenic oilseed plant produces mature seeds in which the total seed fatty acid profile comprises at least 10.0% arachidonic acid and less than 1% total omega-3 fatty acids having at least eighteen carbon atoms and at least four double bonds.
3 more elements
Dependencies are inferred from the claim text.