Methods for producing antibodies
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsTatsuhiko Kodama, Kou-ichi Jishage, Nobuo Kamada, Yoshiki Yamada
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Abstract
This invention provides methods for producing antibodies, wherein the methods comprise the step of administering an immunogen comprising both a target antigen and a background antigen to transgenic animals, into which a gene coding for the background antigen has been introduced. Since immunotolerance to the background antigens have thus been induced in the transgenic animals, the animals efficiently produce antibodies to target antigens.
Claims43
A transgenic non-human animal expressing a transgene encoding a baculoviral envelope protein, the transgene being operably linked to a promoter, wherein the animal has immunotolerance for the protein.
A method of generating a transgenic non-human animal that has immunotolerance for a baculoviral envelope protein, the method comprising
- introducing into a non-human animal, during the animal's fetal stage or after birth, a transgene that encodes a baculoviral envelope protein and is operably linked to a promoter, so that the transgene is expressed in the animal during the fetal stage of life and/or after birth;
- and
- confirming that the animal has immunotolerance for the baculoviral envelope protein.
A method for generating a transgenic non-human animal with immunotolerance for a baculoviral envelope protein, the method comprising
- producing a transgenic non-human animal expressing a genomic transgene that encodes a baculoviral envelope protein and is operably linked to a promoter;
- and
- confirming that the animal has immunotolerance for the baculoviral envelope protein.
Dependencies are inferred from the claim text.