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US 8,013,180 B2Granted
Method for immobilizing compound onto column carrier
AssigneeNEC Corporation
InventorsMinoru Asogawa, Toru Matsumoto
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Abstract
It is intended to provide a method for immobilizing, onto a column carrier, a compound having a sulfo group or a compound to which a sulfo group can be added. The method comprises immobilizing the compound onto the column carrier using a linker comprising: a sulfo group-binding group capable of forming a bond with the sulfo group in the compound; and a carrier-binding group capable of forming a bond with a group present on the surface of the column carrier.
Claims4
A method for immobilizing a compound onto a column carrier by using a linker,
- wherein the compound is a sulfonated aromatic compound, and uses an amino acid as the linker,
- the method comprising:
- a first step of forming a bond between the compound and the amino acid by a reaction between a sulfonate group of the compound and an amino group of the amino acid, and,
- a second step of forming a bond between carboxy group of the amino acid and a group present on the surface of the column carrier.
1 more element
Dependencies are inferred from the claim text.