hnRNP K expression-inhibiting compound and siRNA sequence thereof
AssigneeChang Gung University
InventorsLih-Chyang Chen, Yu-Sun Chang
Life of this patent
Estimating the term…
Abstract
The present invention discloses an hnRNP K expression-inhibiting compound and a siRNA sequence thereof, wherein a siRNA sequence partially or completely complementary to the sequence of hnRNP K is used to inhibit hnRNP K expression, whereby is effectively reduced the survival rate of cancer cells in an anoxic environment.
Claims5
A heterogeneous nuclear ribonucleoprotein K expression-inhibiting compound, which is a nucleotide-based molecule having a sense region and an antisense region jointly forming a duplex region, wherein each of said sense region and said antisense region has a length of 18-30 nucleotides, and wherein said antisense region containing a sequence matching with a sequence of an mRNA of heterogeneous nuclear ribonucleoprotein K and inhibiting expression of heterogeneous nuclear ribonucleoprotein K and suppress growth, metastasis and invasion of cancer cells,
- wherein a sequence of said sense region is expressed by
- UAAACGCCCUGCAGAAGAUUU
- (SEQ ID NO:
- 1)
- GGUCGUGGCUCAUAUGGUGUU
- (SEQ ID NO:
- 2)
- UGACAGAGUUGUUCUUAUUUU
- (SEQ ID NO:
- 3)
- GCAAGAAUAUUAAGGCUCUUU
- (SEQ ID NO:
- 4).
A heterogeneous nuclear ribonucleoprotein K expression-inhibiting small interfering ribonucleic acid sequence, which is expressed by
- UAAACGCCCUGCAGAAGAUUU
- (SEQ ID NO:
- 1)
- GGUCGUGGCUCAUAUGGUGUU
- (SEQ ID NO:
- 2)
- UGACAGAGUUGUUCUUAUUUU
- (SEQ ID NO:
- 3)
- GCAAGAAUAUUAAGGCUCUUU
- (SEQ ID NO:
- 4)
- and which can suppress growth, metastasis and invasion of cancer cells via inhibiting expression of heterogeneous nuclear ribonucleoprotein K.
Dependencies are inferred from the claim text.