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US 8,013,134 B2Granted
Kit for proximity probing with multivalent proximity probes
AssigneeOlink AB
InventorSimon Fredriksson
Life of this patent
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Abstract
The present invention relates to sensitive, rapid and convenient assays for detection and or quantification of one or more analyte(s) in solution using multivalent proximity probes. The proximity probes each comprise several binding moieties, such as antibodies, and associated nucleic acid(s). When the binding moieties have bound to their analyte(s), the nucleic acids on opposite proximity probes interact with each other and a signal is generated based on this interaction. The multivalent proximity probes are especially valuable for highly sensitive and specific protein detection.
Claims18
A kit comprising:
- a pair of different streptavidin-oligonucleotide conjugates, each of said conjugates comprising an oligonucleotide and a streptavidin that is not associated with a biotinylated molecule, wherein one of the conjugates of said pair has an oligonucleotide with a free 3′ end and the other member of the conjugates of said pair has an oligonucleotide with a free 5′ end;
- and
- a splint ligation template oligonucleotide having a sequence that can hybridize to both of the oligonucleotides of said pair of conjugates when the oligonucleotides of said pair of conjugates are in proximity to one another and in contact with said splint ligation template oligonucleotide.
A kit comprising:
- a pair of different streptavidin-oligonucleotide conjugates, each of said conjugates comprising an oligonucleotide and a streptavidin that is not associated with a biotinylated molecule, wherein the oligonucleotide in one of said pair of conjugates is different from the oligonucleotide in the other member of said pair of conjugates;
- a splint ligation template oligonucleotide having a sequence that can hybridize to both of the oligonucleotides of said pair of conjugates when the oligonucleotides of said pair of conjugates are in proximity to one another and in contact with said splint ligation template oligonucleotide;
- and
- a pair of PCR primers, one of said pair of PCR primers having a sequence that can hybridize to the oligonucleotide of one of said pair of conjugates and the other member of said pair of PCR primers having a sequence that can hybridize to the oligonucleotide of the other member of said pair of conjugates.
1 more element
Dependencies are inferred from the claim text.