Synthetic membrane anchors
AssigneeKode Biotech Limited
InventorsNicolai Bovin, Lissa Gilliver, Stephen Henry, Elena Korchagina
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Abstract
The invention relates to synthetic molecules that spontaneously and stably incorporate into lipid by-layers, including cell membranes. Particularly, although not exclusively, the invention relates to the use of these molecules as synthetic membrane anchors or synthetic molecule constructs to effect qualitative and quantitative changes in the expression of cell surface antigens.
Claims20
A method of incorporating a synthetic molecule construct of the structure F—S1—S2-L into the lipid bi-layer of a cell or a multi-cellular structure including the step:
- of contacting a suspension of the cell or multi-cellular structure with the synthetic molecule construct for a time and at a temperature sufficient to allow incorporation where:
- F is a mono-, di-, tri- or oligo-saccharide;
- S1 is 2-aminoethyl, 3-aminopropyl, 4-aminobutyl, or 5-aminopentyl;
- S2 is —CO(CH2)2CO—, —CO(CH2)3CO—, —CO(CH2)4CO— or —CO(CH2)5CO—;
- and
- L is a diacyl- or dialkyl-glycerophospholipid.
The method according to 1 where the suspension of the cell or multi-cellular structure is contacted with the construct at a temperature in the range 2 to 37° C.
Dependencies are inferred from the claim text.