Protein separation device
AssigneeAgency for Science, Technology and Research
InventorsHeng Hang Tsai, Hon-Chiu Eastwood Leung
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Abstract
The invention provides a protein separation device comprising a chaperone protein immobilized on a substrate. In one embodiment, the chaperone protein is an Hsp60 chaperone, preferably a group one chaperone, preferably GroEL. The invention also provides a method for isolating a protein from a biological sample using a protein separation device of the invention.
Claims30
A protein separation device comprising GroEL immobilised on a substrate, wherein the specificity of GroEL is directed to a particular protein, wherein GroEL is engineered by site-directed mutagenesis to have the substitutions, leucine 200 to arginine;
- serine 201 to glycine, and proline 202 to aspartate.
A protein separation device comprising GroEL immobilised on a substrate, wherein the specificity of GroEL is changed to a protein specificity of another chaperone protein, in which GroEL is engineered by site-directed mutagenesis to have the substitutions, Tyrosine 199 to Isoleucine;
- Tyrosine 204 to Isoleucine;
- Leucine 234 to Isoleucine;
- Leucine 237 to Isoleucine;
- Leucine 259 to Phenylalanine;
- Valine 263 to Leucine and Valine 264 to Phenyalanine.
Dependencies are inferred from the claim text.