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US 8,013,121 B2Granted
Isolated nature-identical collagen
AssigneeFraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
InventorsXin Xiong, Heike Mertsching, Steffen Rupp, Herwig Brunner
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Abstract
The present invention concerns isolated collagen, the method for its production, and isolation of the collagen from collagen-containing tissues, as well as the use of the isolated collagen in a biomatrix as an in vitro test system, tissue replacement or organ replacement.
Claims15
A method for the isolation of collagen or collagen matrix proteins from collagen-containing tissue wherein the only extractant utilized is urea, comprising the steps:
- a) isolation of collagen-containing fibers from the tissues;
- b) incubation of the isolated collagen-containing fibers in an aqueous solution containing an extractant, in which the only extractant in the aqueous solution consists of urea in a final concentration of 5-15 mol/L, in which a collagen-containing fraction is dissolved out of the fibers;
- c) separation of the dissolved collagen-containing fraction from the fiber and tissue residue;
- and
- d) separation of the urea from the collagen-containing fraction and renaturing of the collagen by use of gradient dialysis in aqueous PBS, so that a collagen-containing aqueous solution with isolated collagen is obtained.
2 more elements
Dependencies are inferred from the claim text.