US 8,013,115 B1Granted

Use of stabilised synthetic compounds in immunoassay

AssigneeBio-Rad Pasteur

InventorsIsabelle Nathalie Garric, Isabelle Karine Giuliani, Catherine Christiane Marie Larue, François Yves Rieunier, Sylvie Marie-France Trinquier

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Abstract

The invention concerns the use of at least a synthetic compound comprising: a carrier molecule having at least a hydrocarbon chain comprising at least 10, advantageously at least 12, preferably at least 14 and not more than 300, advantageously not more than 100, preferably not more than 50, selected'among C, N, O, S, P and Si, and comprising at least two reactive functions, or capable of reacting, for grafting lateral arms on said chain, and at least two different epitopes carried by said lateral arms grafted on said carrier molecule, as standard or control for immunoassay of said biological molecules, in particular of polypeptides or proteins.

Claims17

Dependent claims

A synthetic compound consisting of:

a) a cyclic or noncyclic polypeptide carrier molecule backbone, wherein the cyclic polypeptide carrier molecule backbone consists of 8 to 50 amino acid residues and the noncyclic polypeptide carrier molecule backbone consists of 7 to 50 amino acid residues, containing the sequence:
X1-A1-A2-A3-A4-A5-X2-A6
wherein
X1 and X2 are lysine residues,
A1 and A2 represent an amino acid residue selected from the group consisting of proline, phenylalanine, glycine, alanine, valine, leucine, and isoleucine, with the proviso that one of A1 and A2 is selected from the group consisting of proline and phenylalanine,
A3 represents an amino acid residue selected from the group consisting of glycine, alanine, valine, leucine, and isoleucine,
A4 represents an amino acid residue selected from the group consisting of arginine and histidine,
A5 represents an amino acid residue selected from the group consisting of glycine, alanine, valine, leucine, and isoleucine, and
A6 represents an amino acid residue selected from the group consisting of glycine, alanine, valine, leucine, and isoleucine;
and
b) a side arm grafted to the side chain of each of the two X1 and X2 lysine residues, the side arm carrying at least two different epitopes of human cardiac troponin I.

8 more elements

Dependencies are inferred from the claim text.