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US 8,013,114 B2Granted
Fusion proteins based upon somatostatin receptors
AssigneeBoard of Regents, The University of Texas System
InventorVikas Kundra
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Abstract
The present invention is directed to fusion proteins that can be used to assay gene transfer and expression both in vitro and in vivo. The fusion proteins contain a reporter protein, e.g. a somatostatin receptor, fused to a second protein, which may be a protein fusion tag. Alternatively, a fusion protein may be fused to a leader sequence. A leader sequence may localize an expressed protein, e.g localize a fusion protein to the cell membrane. The invention includes nucleic acids encoding the fusion proteins and methods of assaying for gene expression.
Claims17
A fusion protein comprising:
- a) a somatostatin receptor polypeptide, the somatostatin receptor polypeptide being truncated at its C-terminus relative to wild-type somatostatin receptor;
- and
- b) a heterologous polypeptide fused to the N-terminus, if not truncated, or the trucated N-terminus, if truncated, or truncated C-terminus of said somatostatin receptor polypeptide:
- wherein:
- i) the C-terminus of said somatostatin receptor polypeptide is truncated at amino acid 314;
- ii) the somatostatin receptor polypeptide is also truncated at its N-terminus;
- iii) the fusion protein comprises a leader sequence fused to the truncated C-terminus of said somatostatin receptor polypeptide;
- iv) the heterologous polypeptide is a protein fusion tag that is hemagglutinin A or has enzymatic activity;
- or
- v) the fusion protein comprises a leader sequence that is an IgK leader sequence.
7 more elements
Dependencies are inferred from the claim text.