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US 8,013,009 B2Granted
Methods of diagnosing infertility by detecting PMCA4 mutations
OwnerLudwig Neysesinventor
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Abstract
The invention relates to the use of plasma membrane calcium ATPase (PMCA) inhibitors for inhibiting sperm mobility to achieve a contraceptive effect. The invention further relates to contraceptive agents comprising one or more PMCA inhibitors.
Claims6
A method for diagnosing infertility in a human male, comprising:
- obtaining a biological sample from the human male, wherein the biological sample contains one or more sperm cells;
- analyzing the biological sample, wherein
- (i) detecting a mutation or polymorphism in a plasma membrane calcium ATPase 4 (PMCA4) gene encoding PMCA4 in the one or more sperm cells, or
- (ii) detecting a decrease in the expression of PMCA4 in the one or more sperm cells relative to a control sample,
- is diagnostic of infertility;
- and
- counting a number of non-motile sperm cells relative to motile sperm cells in the biological sample, wherein a number of non-motile sperm cells is greater than 30%.
4 more elements
A method for diagnosing infertility in a human male, comprising:
- obtaining a biological sample from the human male, wherein the biological sample contains one or more sperm cells;
- analyzing the biological sample, wherein
- (i) detecting a mutation or polymorphism in a plasma membrane calcium ATPase 4 (PMCA4) gene encoding PMCA4 in the one or more sperm cells, wherein the mutation or polymorphism is detected in exon 2 or exon 3 of the PMCA4 gene or
- (ii) detecting a decrease in the expression of PMCA4 in the one or more sperm cells relative to a control sample,
- is diagnostic of infertility;
- and counting a number of non-motile sperm cells relative to motile sperm cells in the biological sample, wherein a number of non-motile sperm cells is greater than 30%.
3 more elements
Dependencies are inferred from the claim text.