Phage antibodies to radiation-inducible neoantigens
AssigneeVanderbilt University
InventorsDennis E. Hallahan, Raymond Mernaugh
Life of this patent
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Abstract
A method for identifying a molecule that binds an irradiated tumor in a subject and molecules identified thereby. The method includes the steps of: (a) exposing a tumor to ionizing radiation; (b) administering to a subject a library of diverse molecules; and (c) isolating from the tumor one or more molecules of the library of diverse molecules, whereby a molecule that binds an irradiated tumor is identified. Also provided are therapeutic and diagnostic methods using targeting ligands that bind an irradiated tumor.
Claims13
An immunoconjugate composition comprising a single chain fragment variable (scFv) antibody that binds to a radiation-inducible neoantigen, or a Fab fragment thereof, wherein the single chain fragment variable (scFv) antibody:
- (i) is encoded by a nucleic acid encoding an scFv antibody having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 18, 20, 22, and 24;
- (ii) is encoded by a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 17, 19, 21, and 23;
- or
- (iii) has an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 18,20,22, and 24.
A polyvalent immunoconjugate composition comprising a plurality of antibodies or antibody fragments selected from the group consisting of single chain fragment variable (scFv) antibodies, Fab antibodies, and combinations thereof, wherein;
- (i) the plurality of antibodies or antibody fragments bind to a plurality of different epitopes, at least one of which is present on a radiation-inducible neoantigen;
- and
- (ii) at least one of the plurality of antibodies is a single chain fragment variable (scFv) antibody that:
- (1) is encoded by a nucleic acid encoding an scFv antibody having an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 18, 20, 22, and 24;
- (2) is encoded by a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 17, 19, 21, and 23;
- or
- (iii) has an amino acid sequence selected from the group consisting of SEQ ID NOS:
- 18, 20, 22, and 24.
Dependencies are inferred from the claim text.