Truncated PAP2 and methods of making and using same

A method of treating pancreatitis, comprising:

• providing a mammal having pancreatitis;
• and
• administering a therapeutically effective amount of truncated pancreatitis associated protein of the sequence listing
• (SEQ ID NO:
• 1)
• LVTTLKSWFQADLACQKRPSGHLVSILSGGEASFVSSLVTGRVNNNQDIWI
• WLHDPTMGQQPNGGGWEWSNSDVLNYLNWDGDPSSTVNRGNCGSLTATSEF
• LKWGDHHCDVELPFVCKFKQ.

A method of providing an immunomodulatory effect in a mammal with pancreatitis, comprising:

• administering to the mammal in need thereof a therapeutically effective amount of a truncated pancreatitis associated protein of the sequence listing
• (SEQ ID NO:
• 1)
• LVTTLKSWFQADLACQKRPSGHLVSILSGGEASFVSSLVTGRVNNNQDIWI
• WLHDPTMGQQPNGGGWEWSNSDVLNYLNWDGDPSSTVNRGNCGSLTATSEF
• LKWGDHHCDVELPFVCKFKQ.

A method of making recombinant truncated pancreatitis-associated protein of the sequence listing

• (SEQ ID NO:
• 1)
• LVTTLKSWFQADLACQKRPSGHLVSILSGGEASFVSSLVTGRVNNNQDIWI
• WLHDPTMGQQPNGGGWEWSNSDVLNYLNWDGDPSSTVNRGNCGSLTATSEF
• LKWGDHHCDVELPFVCKFKQ,
• comprising the steps of:
• Inserting a nucleic acid encoding SEQ ID NO:1 in-frame into a pET24a bacterial expression vector;
• growing a plurality of positive clones transformed into a plurality of bacterium to a density in a medium;
• centrifuging and resuspending the bacterium in a resuspension buffer;
• sonicating the bacterium with a protease inhibitor to lyse the cells;
• centrifuging and washing the bacterial cell lysate pellet in a first buffer;
• solubilizing said bacterial cell lysate pellet in a second buffer containing 6 M urea.