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US 8,012,764 B2Granted
Mass spectrometer
AssigneeMicromass UK Limited
InventorsRichard Denny, Keith Richardson, John Skilling
Life of this patent
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Abstract
A mass spectrometer and method of mass spectrometry are disclosed wherein two separate samples are mass analysed and then the relative intensity, concentration or expression level of one or more components, molecules or analytes in a first sample is quantitated relative to the intensity, concentration or expression level of one or more components, molecules or analytes in a second sample. The relative quantitation is performed probabilistically without the need to resort to using internal calibrants.
Claims26
A method of mass spectrometry comprising:
- providing a first sample comprising a first mixture of components, molecules or analytes;
- providing a second different sample comprising a second mixture of components, molecules or analytes;
- and
- probabilistically determining or quantifying the relative intensity, concentration or expression level of a component, molecule or analyte in said first sample relative to the intensity, concentration or expression level of a component, molecule or analyte in said second sample;
- wherein said first sample and said second sample comprise a plurality of different biopolymers, proteins, protein digest products, peptides, fragments of peptides, polypeptides, oligionucleotides, oligionucleosides, amino acids, carbohydrates, sugars, lipids, fatty acids, vitamins, hormones, portions or fragments of DNA, portions or fragments of cDNA, portions or fragments of RNA, portions or fragments of mRNA, portions or fragments of tRNA, polyclonal antibodies, monoclonal antibodies, ribonucleases, enzymes, metabolites, polysaccharides, phosphorolated peptides, phosphorolated proteins, glycopeptides, glycoproteins or steroids.
2 more elements
Dependencies are inferred from the claim text.