Antibody compositions
AssigneeGenentech, Inc.
InventorsErnst H. Rinderknecht, Gerardo A. Zapata
Life of this patent
Estimating the term…
Abstract
A process for purifying an antibody is provided. In this process, a mixture containing the antibody and a contaminant is subjected to low pH hydrophobic interaction chromatography (LPHIC) at low salt concentration. The antibody is eluted from the column in the fraction which does not bind thereto. This process can be preceded and followed by other purification steps.
Claims17
A composition comprising a mixture of isolated incorrectly disulfide linked antibody fragments and correctly linked disulfide linked antibody fragments, wherein the purity of the correctly linked disulfide antibody in the composition is at least 95%, wherein the antibody fragment is selected from the group consisting of:
- Fab, Fab′, F(ab′)2, and L-F(ab′)2.
Dependencies are inferred from the claim text.