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US 8,012,747 B2Granted
Expression system
AssigneeSan Diego State University Foundation
InventorJacques Perrault
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Abstract
The present invention relates generally to methods and compositions for expression of polypeptides or delivery of interfering RNA's in various cell types.
Claims8
A recombinant vesicular stomatitis virus vector expression system comprising:
- (a) a vesicular stomatitis virus vector particle (VSV) comprising a polynucleotide encoding a T7 RNA polymerase, wherein when the vector particle is used to infect a cell, the T7 polymerase is expressed in the cell;
- and
- (b) a recombinant plasmid vector comprising a polynucleotide comprising the following elements operably linked 5′ to 3′:
- (i) a T7 promoter, and
- (ii) a heterologous gene, wherein the heterologous gene comprises a sequence encoding an internal ribosome entry site (IRES), wherein when the recombinant plasmid vector is used to transfect said cell, a polypeptide is expressed from transcripts encoded by said heterologous gene in the cell.
2 more elements
A recombinant vesicular stomatitis virus vector expression system comprising:
- (a) a vesicular stomatitis virus vector particle (VSV) comprising a polynucleotide encoding a bacteriophage RNA polymerase, wherein when the vector particle is used to infect a cell, the polymerase is expressed in the cell;
- and
- (b) a recombinant plasmid vector comprising a polynucleotide comprising the following elements operably linked 5′ to 3′:
- (i) a bacteriophage promoter corresponding to said bacteriophage RNA polymerase, and
- (ii) a heterologous gene, wherein the heterologous gene comprises a sequence encoding an internal ribosome entry site (IRES), wherein when the recombinant plasmid vector is used to transfect said cell, a polypeptide is expressed from transcripts encoded by said heterologous gene in the cell.
2 more elements
Dependencies are inferred from the claim text.