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US 8,012,736 B2Granted
Multi plasmid system for the production of influenza virus
AssigneeMedImmune, LLC
InventorsErich Hoffman, Hong Jin, Bin Lu, Greg Duke, George Kemble
Life of this patent
Estimating the term…
Abstract
Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided.
Claims8
A helper virus free method for producing infectious influenza B viruses in cell culture, the method comprising:
- i) electroporating a population of Vero cells with a plurality of plasmids comprising nucleic acid sequences corresponding to an influenza B virus genome;
- ii) culturing the population of Vero cells under conditions permissive for viral replication in the absence of helper virus;
- and,
- iii) recovering a plurality of infectious influenza B viruses.
1 more element
A helper virus free method for producing infectious influenza B viruses in cell culture, the method comprising:
- i) electroporating a population of Vero cells with eight plasmids comprising nucleic acid sequences corresponding to an influenza B virus genome encoding an attenuated, cold adapted, temperature sensitive influenza virus;
- ii) culturing the population of Vero cells under conditions permissive for viral replication in the absence of helper virus at a temperature less than or equal to 35° C. and in serum free medium;
- and,
- iii) recovering a plurality of infectious influenza B viruses.
1 more element
Dependencies are inferred from the claim text.