Thrombin preparations and process for their production
AssigneeCSL Behring GmbH
InventorsHubert Metzner, Heinrich Scheider
Life of this patent
Estimating the term…
Abstract
The production of a thrombin preparation which is obtained from prothrombin which is, after activation to thrombin without the addition of thromboplastin, purified by a hydrophobic interaction chromatography, it being possible subsequently also to inactivate or remove viruses, is described. Before or after the hydrophobic interaction chromatography it is also possible in addition to carry out a cation exchange chromatography. A thrombin preparation which contains as stabilizer a noncovalently binding inhibitor and to which further stabilizers can be added for stabilization in the liquid state is additionally described.
Claims10
A process for producing a thrombin preparation, which comprises obtaining prothrombin from plasma or a plasma fraction, performing ion exchange chromatography on the prothrombin, activating the prothrombin to thrombin, performing hydrophobic interaction chromatography on the activated thrombin, and performing cation exchange chromatography on the hydrophobic interaction chromatography eluate to yield said thrombin preparation, wherein the hydrophobic interaction chromatography eluate is bound directly to the cation exchange chromatography medium and eluted with a salt gradient, and wherein the yield of thrombin in the thrombin preparation is at least about 70%.
Dependencies are inferred from the claim text.