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US 8,012,723 B2Granted
Process for the production of macrolides using a novel strain, Streptomyces sp. BICC 7522
AssigneeBiocon Limited
InventorsMadhav Kulkarni, Surekha K. Prabhu, Madenahally Channabasappa Shivakumar, Prijyajit Sengupta, Sanjay Tiwari, Rakesh Mendhe, Nitin Patil, Laxmi Adhikary, Anand Khedkar, Ramakrishnan Melarkode, Ramavana Gururaja, Shrikumar Suryanarayan
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Abstract
The present invention discloses a new strain of Streptomyces sp. BICC 7522, its variants or mutants and use of the strain for the production of macrolides, process of production and purification of macrolides.
Claims31
A process for the production of a macrolide, the process comprising the step of:
- (a) employing Streptomyces sp. BICC 7522;
- (b) fermenting Streptomyces BICC 7522;
- (c) isolating the macrolide from a broth or an aqueous phase, said isolating step comprising the steps of:
- extracting with a water immiscible organic solvent, and
- concentrating the macrolide in the organic phase;
- (d) treating the organic phase containing macrolide to precipitate impurities;
- (e) separating the precipitated impurities from the organic phase;
- and
- (f) purifying the macrolide,
- wherein the macrolide is Tacrolimus.
7 more elements
The process of 28 wherein the buffer comprises KH2PO4, triethyl amine, and phosphoric acid.
Dependencies are inferred from the claim text.