Method for producing L-amino acid
AssigneeAjinomoto Co., Inc.
InventorsAkito Chinen, Hisashi Yasueda, Jun Nakamura
Life of this patent
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Abstract
L-amino acids such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, and L-cysteine are produced by culturing in a medium a bacterium having an L-amino acid-producing ability and wherein the bacterium has been modified so that the phosphotransacetylase activity is enhanced.
Claims8
A method for producing an L-amino acid comprising culturing in a medium an L-amino acid-producing bacterium which has been modified to enhance phosphotransacetylase activity, and collecting the L-amino acid from the medium or the bacterium, wherein said phosphotransacetylase is encoded by a DNA selected from the group consisting of:
- (a) a DNA comprising nucleotides 1214 to 2641 of SEQ ID:
- 34,
- (b) a DNA comprising the nucleotide sequence of SEQ ID NO:
- 40,
- (c) a DNA which hybridizes with a nucleotide sequence which is complementary to the nucleotide sequence of nucleotides 1214 to 2641 of SEQ ID NO:
- 34, where said DNA hybridizes under stringent conditions and encodes a protein that has phosphotransacetylase activity, and
- (d) a DNA which hybridizes with a nucleotide sequence which is complementary to the nucleotide sequence of SEQ ID NO:
- 40, wherein said DNA hybridizes under stringent conditions and encodes a protein that has phosphotransacetylase activity,
- wherein said stringent conditions comprise washing at a salt concentration of 0.1×SSC, 0.1% SDS at 60° C.
Dependencies are inferred from the claim text.