Method for determination of allergen in environment and kit for simple quantification of allergen
AssigneeJapan Science and Technology Agency
InventorsAki Iwasaki, Koji Suzuki
Life of this patent
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Abstract
A simple method for measuring allergens is disclosed, by which the amount of environmental allergens can be measured simply without using an anti-allergen antibody. In the method for measuring an environmental biological allergen(s), a solution containing a substrate of a protease which the allergen(s) has (have) is brought into contact with a test sample collected by using an adhesive sheet, which substrate gives a visible color change as a result of an enzyme reaction; and measuring the protease activity in the test sample using the color change of the substrate solution as an index, thereby measuring the biological allergen(s).
Claims12
A method for measuring an environmental biological allergen(s), characterized in that said method comprises the steps of immersing a test sample collected by using an adhesive sheet in a solution containing a substrate of a protease which said allergen(s) has(have), in the state of being adhered to said adhesive sheet without a pretreatment, said substrate' being Boc-Val-Leu-Lys-3RAX and/or Boc-amino acid-3RAX (wherein in these formulae, Boc represents t-butyloxycarbonyl group which is a protective group of amino group, and 3RAX represents methylene violet 3RAX);
- and detecting the protease activity in said test sample using a color change of said substrate solution as an indicator of the presence and/or quantity of the environmental biological allergen(s), thereby detecting said biological allergen(s), wherein said allergen(s) to be measured are at least one selected from the group consisting of mite and materials originating from mite.
A method for measuring an environmental biological allergen(s) related to dust mites, characterized in that said method comprises the steps of:
- immersing a test sample comprising dust collected by using an adhesive sheet in a solution containing a substrate of a protease of dust mites, in the state of being adhered to said adhesive sheet without a pretreatment, said substrate being Boc-Val-Leu-Lys-3RAX and/or Boc-amino acid-3RAX (wherein in these formulae, Boc represents t-butyloxycarbonyl group which is a protective group of amino group, and 3RAX represents methylene violet 3RAX);
- and
- detecting the protease activity in said test sample using a color change of said substrate solution as an indicator of the presence and/or quantity of the environmental biological allergen(s), thereby detecting said biological allergen(s).
Dependencies are inferred from the claim text.