H+-gated ion channel
AssigneeUniversity of Utah Research Foundation
InventorsErik M. Jorgensen, Asim A. Beg, Paola Nix
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Abstract
The invention relates to an isolated or recombinant Na+/H+ exchanger comprising an isolated or recombinant Na+/H+ exchanger, particularly to the PBO-4 Na+/H+ exchanger. Also disclosed is an isolated or recombinant protein component of an H+-gated channel which can be affected by extracellular Ca2+ concentration. In particular, the invention relates to PBO-5 and/or PBO-8 and/or a H+-gated channel composed of PBO-5 and PBO-8. The invention relates to compounds isolated from a vertebrate organism, wherein said compounds comprise at least a part of a H+-gated channel or Na+/H+ exchanger. The invention also relates to a method for identifying a component of a H+-gated channel in a vertebrate organism.
Claims3
A method of screening a candidate compound, comprising:
- (a) introducing a nucleic acid sequence encoding a sequence having at least 80% identity to PBO-5 (SEQ ID NO:
- 2) or PBO-8 (SEQ ID NO:
- 3 or SEQ ID NO:4) into a host cell;
- (b) expressing the nucleic acid sequence to produce a ligand-gated cation channel in the host cell;
- (c) contacting the host cell with a candidate compound;
- and
- (d) screening for activation or inhibition of the ligand-gated cation channel by the candidate compound.
Dependencies are inferred from the claim text.