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US 8,012,701 B2Granted
Agonists of bitter taste receptors and uses thereof
AssigneeDeutsches Institut Fur Ernahrungsforschung Potsdam-Rehbrucke Stiftung Des Offentlichen Rechts
InventorsBernd Bufe, Wolfgang Meyerhof, Christina Kuhn
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Abstract
The present invention relates to agonists of the human bitter-taste receptor hTAS2R3 and its role in bitter taste transduction. The invention also relates to assays for screening molecules that modulate, e.g. suppress or block hTAS2R3 bitter taste transduction or bitter taste response.
Claims17
A method for isolating an agonist or antagonist of bitter taste receptor activity comprising:
- (a) contacting:
- (i) a polypeptide with a potential antagonist;
- or
- (ii) a cell expressing a polypeptide with a potential antagonist;
- wherein said polypeptide according to (i) or (ii) has bitter taste receptor activity and wherein said polypeptide according to (i) or (ii):
- (1) comprises SEQ ID NO:
- 4;
- or
- (2) comprises a polypeptide containing only conservative amino acid substitutions in the amino acid sequence of SEQ ID NO:
- 4, said conservative amino acid substitutions numbering 15 or fewer conservative amino acid substitutions in the amino acid sequence of SEQ ID NO:
- 4;
- and
- (b) determining whether the potential antagonist antagonizes the bitter taste receptor activity of said polypeptide;
- wherein prior, concomitantly and/or after step (a) said polypeptide according to (i) or said cell according to (ii) is contacted with an agonist selected from the group consisting of chloramphenicol, humulone, diphenylthiourea, picrotoxinin and agonistic derivatives thereof.
11 more elements
Dependencies are inferred from the claim text.